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The Wistar Institute

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression
Ling Li 1, Mizuho Fukunaga-Kalabis 1, Meenhard Herlyn 1
1Molecular and Cellular Oncogenesis Program, The Wistar Institute

In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.

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Medicine

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Ling Li 1, Klaus B. Piontek 1, Vivek Kumbhari 1, Masaharu Ishida 2, Florin M. Selaru 1
1Division of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University School of Medicine, 2Department of Surgery, Tohoku University

Bile fluid is a valuable source of extracellular vesicles/exosomes that contain potentially important biomarkers. This protocol represents a robust method to isolate exosomes from human bile for further analyses including miRNA profiling.

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Cancer Research

A Melanoma Patient-Derived Xenograft Model
Min Xiao 1, Vito W. Rebecca 1, Meenhard Herlyn 1
1Wistar Institute

Patient-derived xenograft (PDX) models more robustly recapitulate melanoma molecular and biological features and are more predictive of therapy response compared to traditional plastic tissue culture-based assays. Here we describe our standard operating protocol for the establishment of new PDX models and the characterization/experimentation of existing PDX models.

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Immunology and Infection

Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery
Elizabeth Gregorio 1, Eric Ramirez-Salazar 1, Kristen DeRosa 1, Robin Choi 1, Ling Li 1, Meenhard Herlyn 1
1The Wistar Institute

Humanized mouse models provide a more accurate representation of the human immune microenvironment. This manuscript describes the process in which these models are created through a renal graft of human thymus, injection of human CD34+ cells, and the targeted delivery of human cytokine transgenes to promote CD34+ cell proliferation and differentiation.

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Biology

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
Min Li *1, Hongkun Qin *1, Yunfei Long 1, Meiqin Cheng 1, Ling Li 1, Aijun Huang 2, Nian Wang 3, Shuo Duan 1
1China-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center, Gannan Normal University, 2Department of Biological Sciences, Gannan Normal University, 3Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida - Institute of Food and Agricultural Sciences

In this work, a rapid, sensitive, and portable detection method for Candidatus Liberibacter asiaticus based on recombinase polymerase amplification combined with CRISPR-Cas12a was developed.

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