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Universiti Sains Malaysia

3 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Peptide-based Identification of Functional Motifs and their Binding Partners
Martin N. Shelton 1,2, Ming Bo Huang 1, Syed Ali 1,3, Kateena Johnson 1, William Roth 1, Michael Powell 1, Vincent Bond 1
1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia

Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.

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Medicine

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
Simone S. E. Nielsen 1, Piotr Siupka 1, Ana Georgian 2, Jane E. Preston 2, Andrea E. Tóth 1, Siti R. Yusof 2,3, N. Joan Abbott 2, Morten S. Nielsen 1
1Lundbeck Foundation Research Initiative on Brain Barriers and Drug Delivery, Department of Biomedicine, Aarhus University, 2Institute of Pharmaceutical Science, King's College London, 3HICoE Centre for Drug Research, Universiti Sains Malaysia

The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.

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Biology

Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs
Mohammad Asmat-Ullah 1, Rusydi Rozaimi 1, Hanafiah Fazhan 1,2,3, Alexander Chong Shu-Chien 2,4, Youji Wang 5, Khor Waiho 1,2,3
1Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 2Centre for Chemical Biology, Universiti Sains Malaysia, 3Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 4School of Biological Sciences, Universiti Sains Malaysia, 5International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University

Two eyestalk ablation protocols (i.e., cauterization and surgery approaches) were performed on anesthetized female crabs. The eyestalk ablation of mud crabs hastened the ripening of ovaries without decreasing the survival rate.

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