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Children's Healthcare of Atlanta

3 ARTICLES PUBLISHED IN JoVE

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Biology

A Rapid In Vivo Bioassay for Developmentally Active Enhancers
Charmaine U. Pira 1, Shelley A. Caltharp 2, Endika Haro 1, Kerby C. Oberg 1
1Department of Pathology and Human Anatomy, Loma Linda University, 2Department of Pathology, Children's Healthcare of Atlanta

With the increased use of transcription factor-specific ChIP-seq technology, techniques to validate potential regulatory regions are critical. The authors describe a rapid chick bioassay to validate the activity of regulatory sequences functioning during development.

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Biology

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
M. Edward Quach 1,2, Anum K. Syed 1,2, Renhao Li 1,2
1Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, 2Department of Pediatrics, Emory University School of Medicine

We describe an in-solution method to apply uniform shear to platelet surface receptors using cone-plate viscometry. This method may also be used more broadly to apply shear to other cell types and cell-fragments and need not target a specific ligand-receptor pair.

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Neuroscience

Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury
Rowan O. Brothers *1, Sara Bitarafan *2,3, Alyssa F. Pybus 1,3, Levi B. Wood *1,2,3, Erin M. Buckley *1,4,5
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 3Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 4Department of Pediatrics, Emory University School of Medicine, 5Children's Research Scholar, Children's Healthcare of Atlanta

This protocol presents methods to characterize the neuroinflammatory and hemodynamic response to mild traumatic brain injury and to integrate these data as part of a multivariate systems analysis using partial least squares regression.

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