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Children's Healthcare of Atlanta

3 ARTICLES PUBLISHED IN JoVE

Biology

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

Charmaine U. Pira¹, Shelley A. Caltharp², Endika Haro¹, Kerby C. Oberg¹

¹Department of Pathology and Human Anatomy, Loma Linda University, ²Department of Pathology, Children's Healthcare of Atlanta

With the increased use of transcription factor-specific ChIP-seq technology, techniques to validate potential regulatory regions are critical. The authors describe a rapid chick bioassay to validate the activity of regulatory sequences functioning during development.

Biology

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

M. Edward Quach^1,2, Anum K. Syed^1,2, Renhao Li^1,2

¹Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, ²Department of Pediatrics, Emory University School of Medicine

We describe an in-solution method to apply uniform shear to platelet surface receptors using cone-plate viscometry. This method may also be used more broadly to apply shear to other cell types and cell-fragments and need not target a specific ligand-receptor pair.

Neuroscience

Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury

Rowan O. Brothers^*1, Sara Bitarafan^*2,3, Alyssa F. Pybus^1,3, Levi B. Wood^*1,2,3, Erin M. Buckley^*1,4,5

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, ³Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, ⁴Department of Pediatrics, Emory University School of Medicine, ⁵Children's Research Scholar, Children's Healthcare of Atlanta

This protocol presents methods to characterize the neuroinflammatory and hemodynamic response to mild traumatic brain injury and to integrate these data as part of a multivariate systems analysis using partial least squares regression.