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University Of Massachusetts Boston

5 ARTICLES PUBLISHED IN JoVE

Biology

PuraMatrix Encapsulation of Cancer Cells

Adnan O. Abu-Yousif¹, Imran Rizvi^1,2, Conor L. Evans¹, Jonathan P. Celli¹, Tayyaba Hasan^1,3

¹Wellman Center for Photomedicine Massachusetts General Hospital, Harvard Medical School, ²Thayer School of Engineering, Dartmouth College, ³Department of Dermatology, Harvard Medical School

This video demonstrates how to encapsulate and culture cancer cells in PuraMatrix, a commercially available self assembling peptide gel.

Bioengineering

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

Dustin P. Jones^*1, William Hanna^*1, Hamid El-Hamidi¹, Jonathan P. Celli¹

¹Department of Physics, University of Massachusetts Boston

Particle-tracking microrheology can be used to non-destructively quantify and spatially map changes in extracellular matrix mechanical properties in 3D tumor models.

Biology

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Scott M. Paulissen¹, Linda S. Huang¹

¹Department of Biology, University of Massachusetts Boston

Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format.

Developmental Biology

Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

Liu Yang¹, Sayantanee Paul¹, Sarah DuBois-Coyne¹, Phillip Kyriakakis², Alexey Veraksa¹

¹Biology Department, University of Massachusetts Boston, ²Department of Bioengineering, University of California, San Diego

The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).

Medicine

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

Teresa T. Liu^*1,2, Allison C. Rodgers^*3, Tristan M. Nicholson⁴, Jill A. Macoska^5,6, Paul C. Marker^6,7, Chad M. Vezina^6,8, Dale E. Bjorling^6,8, Alejandro Roldan-Alzate^2,9,10, Diego Hernando^10,11, Granville L. Lloyd¹², Timothy A. Hacker³, William A. Ricke^1,6

We describe the use of high frequency ultrasound with contrast imaging as a method to measure bladder volume, bladder wall thickness, urine velocity, void volume, void duration, and urethral diameter. This strategy can be used to assess voiding dysfunction and treatment efficacy in various mouse models of lower urinary tract dysfunction (LUTD).