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Stockholm University

4 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Giselbert Hauptmann¹, Iris Söll¹, Robert Krautz¹, Ulrich Theopold¹

¹Department of Molecular Biosciences, The Wenner-Gren Institute (MBW), Stockholm University

We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.

Chemistry

Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids (BPCA)

Daniel B. Wiedemeier¹, Susan Q. Lang², Merle Gierga³, Samuel Abiven¹, Stefano M. Bernasconi³, Gretchen L. Früh-Green³, Irka Hajdas⁴, Ulrich M. Hanke¹, Michael D. Hilf¹, Cameron P. McIntyre⁴, Maximilian P. W. Scheider¹, Rienk H. Smittenberg⁵, Lukas Wacker⁴, Guido L. B. Wiesenberg¹, Michael W. I. Schmidt¹

¹Department of Geography, University of Zurich, ²Department of Earth and Ocean Sciences, University of South Carolina, ³Department of Earth Sciences, ETH Zurich, ⁴Laboratory of Ion Beam Physics, ETH Zurich, ⁵Department of Geological Sciences, Stockholm University

We present the benzene polycarboxylic acid (BPCA) method for assessing pyrogenic carbon (PyC) in the environment. The compound-specific approach uniquely provides simultaneous information about the characteristics, quantity and isotopic composition (¹³C and ¹⁴C) of PyC.

Biochemistry

Rapid Isolation of the Mitoribosome from HEK Cells

Shintaro Aibara¹, Juni Andréll¹, Vivek Singh¹, Alexey Amunts¹

¹Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.

Bioengineering

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

Sahar Sultan¹, Aji P. Mathew¹

¹Department of Materials and Environmental Chemistry, Stockholm University

The three critical steps of this protocol are i) developing the right composition and consistency of the cellulose hydrogel ink, ii) 3D printing of scaffolds into various pore structures with good shape fidelity and dimensions and iii) demonstration of the mechanical properties in simulated body conditions for cartilage regeneration.