Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization MicroscopyMárton Gélleri 1, Hilmar Strickfaden 2
1Institute of Molecular Biology (IMB), 2Max Planck Institute for Polymer Research
The present protocol describes a method measuring absolute DNA densities within adherent cell nuclei using Voronoi tessellation of single-molecule localization microscopy data, known volume, genome size, and cell cycle stage.
