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suez canal university

3 ARTICLES PUBLISHED IN JoVE

Genetics

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

Ahmed Elaswad^*1,2, Karim Khalil^*1,3, David Cline¹, Patrick Page-McCaw⁴, Wenbiao Chen⁴, Maximilian Michel⁵, Roger Cone⁵, Rex Dunham¹

¹School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, ²Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, ³Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, ⁴Department of Molecular Physiology and Biophysics, Vanderbilt University, ⁵Life Science Institute, University of Michigan

A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.

Biology

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Peibo Wang^*1, Yongcan Zhou^*1,3, Bei Wang², Ahmed Elaswad⁴, Shifeng Wang^1,3, Weiliang Guo^1,3, Dongdong Zhang^1,3

¹State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, ²Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, ³Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, ⁴Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University

Here, we demonstrate the preparation of a high-quality single-cell suspension of tilapia intestine for single-cell sequencing.

Chemistry

Synthesis of Black Seed Oil-loaded-, Alginate-based, pH-sensitive Beads Using Electrospraying Technique

Abul Kalam Azad¹, Mohamad Dayoob², Vinoth Kumarasamy³, Vetriselvan Subramaniyan⁴, Ghadeer M. Albadrani⁵, Muath Q. Al-Ghadi⁶, Mohamed Kamel⁷, Mohamed M. Abdel-Daim^8,9, Mireia Mallandrich Miret¹⁰, Abd Almonem Doolaanea^11,12

¹Faculty of Pharmacy, University College of MAIWP International, ²Faculty of Pharmacy, MAHSA University, ³Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, ⁴Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, ⁵Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, ⁶Department of Zoology, College of Science, King Saud University, ⁷Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, ⁸Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, ⁹Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, ¹⁰Department of Pharmacy, Pharmaceutical Technology and Physical-Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, ¹¹Research and Development, Sabrena Experience, ¹²Alphastar Lab Systems

A technique employing high electric voltage and a targeted, active ingredient-loaded emulsion to fabricate pH-responsive, uniform microbeads is presented.