suez canal university
4 ARTICLES PUBLISHED IN JoVE
Genetics
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
Ahmed Elaswad *1,2, Karim Khalil *1,3, David Cline 1, Patrick Page-McCaw 4, Wenbiao Chen 4, Maximilian Michel 5, Roger Cone 5, Rex Dunham 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.
Biology
Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
Peibo Wang *1, Yongcan Zhou *1,3, Bei Wang 2, Ahmed Elaswad 4, Shifeng Wang 1,3, Weiliang Guo 1,3, Dongdong Zhang 1,3
1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, 2Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, 3Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, 4Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University
Here, we demonstrate the preparation of a high-quality single-cell suspension of tilapia intestine for single-cell sequencing.
Chemistry
Synthesis of Black Seed Oil-loaded-, Alginate-based, pH-sensitive Beads Using Electrospraying Technique
Abul Kalam Azad 1, Mohamad Dayoob 2, Vinoth Kumarasamy 3, Vetriselvan Subramaniyan 4, Ghadeer M. Albadrani 5, Muath Q. Al-Ghadi 6, Mohamed Kamel 7, Mohamed M. Abdel-Daim 8,9, Mireia Mallandrich Miret 10, Abd Almonem Doolaanea 11,12
1Faculty of Pharmacy, University College of MAIWP International, 2Faculty of Pharmacy, MAHSA University, 3Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 4Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, 5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 6Department of Zoology, College of Science, King Saud University, 7Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 8Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, 9Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, 10Department of Pharmacy, Pharmaceutical Technology and Physical-Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 11Research and Development, Sabrena Experience, 12Alphastar Lab Systems
A technique employing high electric voltage and a targeted, active ingredient-loaded emulsion to fabricate pH-responsive, uniform microbeads is presented.
Chemistry
Development and Characterization of Fusidic Acid-Loaded Alginate-Aloe vera Based Hydrogel Film
Mohamad Dayoob 1, Yuki Wong Yee Kee 1, Wan Hamirul Bahrin 2, Bilal Harieth Alrimawi 3, Vinoth Kumarasamy 4, Vetriselvan Subramaniyan 5, Ghadeer M. Albadrani 6, Muath Q. Al-Ghadi 7, Mohamed Kamel 8, Mohamed M. Abdel-Daim 9,10, Abul Kalam Azad 11
1Faculty of Pharmacy, MAHSA University, 2Radiopharmaceutical Technology Group, Medical Technology Division, Malaysia Nuclear Agency, 3Faculty of Pharmacy, Department of Pharmaceutical Sciences, Aqaba University of Technology, 4Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 5Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, 6Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 7Department of Zoology, College of Science, King Saud University, 8Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 9Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, 10Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, 11Faculty of Pharmacy, University College of MAIWP International
A formulation of fusidic acid-loaded hydrogel film was developed with different Aloe vera ratios and characterized in this study.
À PROPOS DE JoVE
Copyright © 2024 MyJoVE Corporation. Tous droits réservés.