Chick ex ovo Culture and ex ovo CAM Assay: How it Really WorksDaniel S. Dohle 1, Susanne D. Pasa 1, Sebastian Gustmann 2, Markus Laub 3, Josef H. Wissler 4, Herbert P. Jennissen 1, Nicole Dünker 2
1Institute for Physiological Chemistry, Department of Biochemical Endocrinology, University of Duisburg-Essen, 2Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, 3Morphoplant GmbH, 4ARCONS Institute for Applied Research and Didactics
The chick chorioallantoic membrane (CAM) is a unique, naturally immunodeficient supportive culture environment to study angiogenesis and tumorigenesis. This video article demonstrates the different steps in chick ex ovo culture, application of potentially angiogenic substances and successful inoculation of tumor cells and tissues on the surface of the CAM.
