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University of Dundee, Dundee, UK

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

Raman M. Das¹, Arwen C. Wilcock¹, Jason R. Swedlow², Kate G. Storey¹

¹Neural Development Group, Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee, UK, ²Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK

Imaging embryonic tissue in real-time is challenging over long periods of time. Here we present an assay for monitoring cellular and sub-cellular changes in chick spinal cord for long periods with high spatial and temporal resolution. This technique can be adapted for other regions of the nervous system and developing embryo.

Biology

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

John Biddlestone¹, Jimena Druker¹, Alena Shmakova¹, Gus Ferguson¹, Jason R. Swedlow¹, Sonia Rocha¹

¹Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK

We describe a technique for analysis of global RNA synthesis in hypoxia using imaging. Click-chemistry labeling of RNA has not previously been performed under hypoxia and allows visualization of global RNA changes at the single cell level. This approach complements the existing averaged RNA techniques, allowing direct visualization of cell-to-cell changes in global RNA synthesis.