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Karlsruhe Institute of Technology, Karlsruhe, Germany

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

Christine Wittmann¹, Markus Reischl², Asmi H. Shah¹, Ralf Mikut², Urban Liebel^1,2, Clemens Grabher¹

¹Institute for Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany, ²Institute for Applied Informatics, Karlsruhe Institute of Technology, Karlsruhe, Germany

Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.