Facilitating Drug Discovery: An Automated High-content Inflammation Assay in ZebrafishChristine Wittmann 1, Markus Reischl 2, Asmi H. Shah 1, Ralf Mikut 2, Urban Liebel 1,2, Clemens Grabher 1
1Institute for Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany, 2Institute for Applied Informatics, Karlsruhe Institute of Technology, Karlsruhe, Germany
Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.
