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Chungnam National University

6 ARTICLES PUBLISHED IN JoVE

Neuroscience

Automated Gait Analysis in Mice with Chronic Constriction Injury

Dong-Wook Kang¹, Jae-Gyun Choi¹, Ji-Young Moon², Suk-Yun Kang², Yeonhee Ryu², Jin Bong Park¹, Hyun-Woo Kim^1,3

¹Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, ²KM Fundamental Research Division, Korea Institute of Oriental Medicine (KIOM), ³Department of Neuroscience and Cell Biology, University of Texas Medical Branch at Galveston

The precise assessment of pain response in a neuropathic animal model is critical to investigate the pathophysiology of pain diseases and develop new analgesics. We present a sensitive and objective method to determine the sensory function of the rodent hind paw by an automated gait analysis system.

Genetics

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Seoghyun Lee^*1, Minho Won^*2, Ran Hee Hwang³, Gang Min Hur², Hyunju Ro¹

¹Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, ²Department of Pharmacology, College of Medicine, Chungnam National University, ³Department of Nursing, Gwangju Women's University

Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

Immunology and Infection

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

Le Hong Son^*1, Liu Ye^*1, Inkyu Hwang¹

¹College of Pharmacy, Chungnam National University

We have established an ex vivo mast cell degranulation assay carried out by incubating crude peritoneal exudate cells isolated from the mice, treated with a pharmacological agent of interest and administered anti-dinitrophenol (DNP) IgE beforehand, with DNP on a carrier protein.

Neuroscience

Burn Injury-Induced Pain and Depression-Like Behavior in Mice

Jae-Gyun Choi^*1, Dong-Wook Kang^*1, Jaehyuk Kim¹, Miae Lee¹, Sheu-Ran Choi², Jin Bong Park¹, Hyun-Woo Kim¹

¹Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, ²Department of Pharmacology, Catholic Kwandong University College of Medicine

A transient scald injury (65 °C ± 0.5 °C, 3 s) of one hind paw decreases the threshold (g) to von Frey filament stimulation of the ipsilateral side and alters gait pattern. Besides, burn injury induces depression-like behavior in the forced swimming test.

Neuroscience

In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy

Eun-Seo Cho¹, Seungjae Han¹, Gyuri Kim¹, Minho Eom¹, Kang-Han Lee², Cheol-Hee Kim², Young-Gyu Yoon^1,3

¹School of Electrical Engineering, KAIST, ²Department of Biology, Chungnam National University, ³KAIST Institute for Health Science and Technology

Presented here is a protocol for in vivo whole-brain imaging of larval zebrafish using three-dimensional fluorescence microscopy. The experimental procedure includes sample preparation, image acquisition, and visualization.

Biochemistry

Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue

Sanghun Lee^*1, Gyumin Lim^*2,3, Soyeon Kim^*1, Hyoju Kim¹, Yeon Jin Roh³, Wantae Kim², Dong Wook Choi³, Su Myung Jung¹

¹Department of Biological Sciences, Sungkyunkwan University (SKKU), ²Department of Biochemistry, College of Natural Sciences, Chungnam National University, ³Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University

In this protocol, methods relevant for BAT-optimized arteriovenous metabolomics using GC-MS in a mouse model are outlined. These methods allow for the acquisition of valuable insights into BAT-mediated metabolite exchange at the organismal level.