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China Medical University Hospital

4 ARTICLES PUBLISHED IN JoVE

Medicine

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats

Hsin-Ting Lin^1,2, Yi-Lin Shiou¹, Wun-Jyun Jhuang¹, Hsiang-Chun Lee^1,3,4,5,6

¹Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, ²Hematology and Oncology, China Medical University Hospital, ³Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, ⁴Center for Lipid Biosciences, Kaohsiung Medical University Hospital, ⁵Lipid Science and Aging Research Center, Kaohsiung Medical University, ⁶Institute/Center of Medical Science and Technology, National Sun Yat-sen University

Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.

Cancer Research

Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

Chi-Hao Hsiao^1,2,3, Ya-Hsu Chiu⁴, Shao-Chih Chiu^4,5, Der-Yang Cho^4,6,7, Liang-Ming Lee^1,2, Yu-Ching Wen^1,2, Jia-Jhe Li⁴, Ping-Hsiao Shih⁴

¹Department of Urology, Wan Fang Hospital, ²Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, ³Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, ⁴Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, ⁵Graduate Institute of Biomedical Sciences, China Medical University, ⁶Graduate Institute of Immunology, China Medical University, ⁷Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3⁺CD56⁺ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.

Medicine

Fu's Subcutaneous Needling for Knee Osteoarthritis Pain

Po-En Chiu^*1,2, Zhonghua Fu^*3,4, Jian Sun^4,5,6, Guan-Wei Jian^7,8, Te-Mao Li⁹, Li-Wei Chou^10,11,12

We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills.

Medicine

Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model

Po-En Chiu^*1,2, Zhonghua Fu^*3,4, De-Wei Lai^5,6, Li-Wei Chou^7,8,9

¹Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, ²Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, ³Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, ⁴Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, ⁵Experimental Animal Center, Department of Molecular Biology and Cell Research, Chang Bing Show Chwan Memorial Hospital, ⁶Department of Pharmacy and Master Program, Tajen University, ⁷Department of Physical Medicine and Rehabilitation, China Medical University Hospital, ⁸Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, ⁹Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University

We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats.