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H3 Biomedicine

3 ARTICLES PUBLISHED IN JoVE

Biology

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Jian Zou¹, Xiangyun Wei^1,2

¹Department of Ophthalmology, University of Pittsburgh, ²Department of Microbiology and Molecular Genetics, University of Pittsburgh

We demonstrate a protocol to generate chimeric zebrafish embryos for live imaging cellular behavior during embryogenesis.

Medicine

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Manav Korpal¹, Jacob Feala¹, Xiaoling Puyang¹, Jian Zou¹, Alex H. Ramos², Jeremy Wu¹, Timm Baumeister¹, Lihua Yu¹, Markus Warmuth¹, Ping Zhu¹

¹H3 Biomedicine, ²Massachusetts General Hospital

Drug resistance to targeted therapeutics is widespread and the need to identify mechanisms of resistance--prior to or following clinical onset--is critical for guiding alternative clinical management strategies. Here, we present a protocol to couple derivation of drug-resistant lines in vitro with sequencing to expedite discovery of these mechanisms.

Developmental Biology

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Haochuan Zhang^*1,2,3, JunGang Huang^*2,3,4, Xin Liu^2,3, Ping Zhu⁴, Zhongrong Li¹, Xue Li^2,3

¹Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, ²Departments of Urology, Boston Children's Hospital, ³Department of Surgery, Harvard Medical School, ⁴Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences

We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.