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Zhejiang Ocean University

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
Taylor R. Fore 1, Audrey A. Ojwang 1, Margaret L. Warner 1, Xinyun Peng 1, Rudolf A. Bohm 1,2, William P. Welch 1, Lindsey K. Goodnight 1, Hong Bao 1, Bing Zhang 1
1Department of Zoology, University of Oklahoma - Norman, 2Department of Biology, Brandeis University

We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

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Biology

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
Jingwen Yang 1, Yunjun Yan 1, Xiaowei Xiang 1, Yuchao Xu 1, Naiming Zhou 2, Tianming Wang 1
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences, Zhejiang University

This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.

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JoVE Core

Enhanced Electron Injection and Exciton Confinement for Pure Blue Quantum-Dot Light-Emitting Diodes by Introducing Partially Oxidized Aluminum Cathode
Zhibin Wang 1, Tai Cheng 1, Fuzhi Wang 1, Yiming Bai 1, Xingming Bian 1, Bing Zhang 1, Tasawar Hayat 2,3, Ahmed Alsaedi 3, Zhan'ao Tan 1
1State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, 2Department of Mathematics, Quaid-I-Azam University, 3NAAM Research Group, Faculty of Science, King Abdulaziz University

A protocol is presented for fabricating high-performance, pure blue ZnCdS/ZnS-based quantum dots light-emitting diodes by employing an autoxidized aluminum cathode.

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Developmental Biology

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
Jingwen Yang *1,2, Haichun Pan *2, Yuji Mishina 2
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan

Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining.

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Biology

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells
Tianming Wang 1, Xu Chen 1, Ke Xu 1, Bing Zhang 1, Dexiang Huang 1, Jingwen Yang 1
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University

This protocol provides an easy-to-handle method to culture the intestinal cells from sea cucumber Apostichopus japonicus and is compatible with a variety of widely available tissue samples from marine organisms including Echinodermata, Mollusca, and Crustacea.

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