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Zhejiang Ocean University

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Taylor R. Fore¹, Audrey A. Ojwang¹, Margaret L. Warner¹, Xinyun Peng¹, Rudolf A. Bohm^1,2, William P. Welch¹, Lindsey K. Goodnight¹, Hong Bao¹, Bing Zhang¹

¹Department of Zoology, University of Oklahoma - Norman, ²Department of Biology, Brandeis University

We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

Biology

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

Jingwen Yang¹, Yunjun Yan¹, Xiaowei Xiang¹, Yuchao Xu¹, Naiming Zhou², Tianming Wang¹

¹National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, ²Institute of Biochemistry, College of Life Sciences, Zhejiang University

This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.

JoVE Core

Enhanced Electron Injection and Exciton Confinement for Pure Blue Quantum-Dot Light-Emitting Diodes by Introducing Partially Oxidized Aluminum Cathode

Zhibin Wang¹, Tai Cheng¹, Fuzhi Wang¹, Yiming Bai¹, Xingming Bian¹, Bing Zhang¹, Tasawar Hayat^2,3, Ahmed Alsaedi³, Zhan'ao Tan¹

¹State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, ²Department of Mathematics, Quaid-I-Azam University, ³NAAM Research Group, Faculty of Science, King Abdulaziz University

A protocol is presented for fabricating high-performance, pure blue ZnCdS/ZnS-based quantum dots light-emitting diodes by employing an autoxidized aluminum cathode.

Developmental Biology

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Jingwen Yang^*1,2, Haichun Pan^*2, Yuji Mishina²

¹The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, ²Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan

Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining.

Biology

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Tianming Wang¹, Xu Chen¹, Ke Xu¹, Bing Zhang¹, Dexiang Huang¹, Jingwen Yang¹

¹National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University

This protocol provides an easy-to-handle method to culture the intestinal cells from sea cucumber Apostichopus japonicus and is compatible with a variety of widely available tissue samples from marine organisms including Echinodermata, Mollusca, and Crustacea.