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Kwangwoon University

4 ARTICLES PUBLISHED IN JoVE

Biology

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca²⁺ Measurement with Fura-2-Analog in Live Cells

Jeong Hoon Lee¹, Jeong Mi Ha¹, Quynh Mai Ho¹, Chae Hun Leem^1,2,3

¹Department of Physiology, University of Ulsan College of Medicine/Asan Medical Center, ²Asan Medical Center, ³Asan Medical Institute of Convergence Science and Technology

Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca²⁺]. Thus, a novel online correction method of NADH signal interference to measure [Ca²⁺] was developed.

Engineering

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

Philippe Gentet¹, Yves Gentet², Lyoung-Hui Kim¹, Kwang-Jib Kim¹, Seung-Hyun Lee³

¹Department of Plasma Bio Display, Kwangwoon University, ²Bordeaux Holography, ³Department of Ingenium College, Kwangwoon University

We present a protocol to record a set of ultra-realistic full-color analog holograms, showing the same brightness, transparency, and homogeneous colors, on ultra-fine-grain silver-halide holographic emulsions for the fabrication of a dynamic holographic 3D display.

Biochemistry

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Dohwan Lee^*1, Kyung Wook Wee^*1, Hyerin Kim^*1, Sung Il Han¹, Seungmin Kwak^1,2, Dae Sung Yoon³, Jeong Hoon Lee¹

¹Department of Electrical Engineering, Kwangwoon University, ²Korea Institute of Science and Technology (KIST), ³School of Biomedical Engineering, Korea University

Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes.

Bioengineering

High-Performance Graphene-Modified Sensing Chip for SARS-CoV-2 Detection

Parshant Kumar Sharma^1,2,3, Ebrahim Mostafavi^4,5, Nam-Young Kim^1,2,3, Thomas J. Webster⁶, Ajeet Kaushik^7,8

¹RFIC Bio Centre, Kwangwoon University, ²Department of Electronics Engineering, Kwangwoon University, ³NDAC Centre, Kwangwoon University, ⁴Stanford Cardiovascular Institute, Stanford University School of Medicine, ⁵Department of Medicine, Stanford University School of Medicine, ⁶Department of Biomedical Engineering, Hebei University of Technology, ⁷NanoBioTech Laboratory, Department of Environmental Engineering, Florida Polytechnic University, ⁸School of Engineering, University of Petroleum and Energy Studies (UPES)

The present protocol describes the fabrication of low-cost biosensing prototypes based on useful nanosystems for accurately detecting viral proteins (at the Fg level). Such a tiny sensor platform allows for point-of-care applications that can be integrated with the Internet of Medical Things (IoMT) to meet telemedicine objectives.