UV excitable plural probes, have an inherent signal contamination, because of the auto-plural sounds. Most of the problem with NADH, is limiting the use of a plural probe. A method can be used, to precisely correct the outer florescence from NADH,
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Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca2+]. Thus, a novel online correction method of NADH signal interference to measure [Ca2+] was developed.