NYU School of Medicine
2 ARTICLES PUBLISHED IN JoVE
Medicine
Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
Stephen T. Armenti 1, Jonathan P. Greenberg 2, R. Theodore Smith 1
1Department of Ophthalmology, NYU School of Medicine, 2Harkness Eye Institute, Columbia University
The retinal pigment epithelium (RPE) supports the sensory retina through recycling visual cycle byproducts, which accumulate as lipofuscin. These products are autofluorescent and can be qualitatively imaged in vivo. Here, we describe a method to quantitatively image RPE lipofuscin using confocal scanning laser ophthalmoscopy.
Developmental Biology
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle
Wenyue Guan 1, Lalanti Venkatasubramanian 2, Myungin Baek 3, Richard S. Mann 2, Jonathan Enriquez 1
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, 3Neuroscience Program, NYU School of Medicine
Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.
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