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korea research institute of bioscience and biotechnology

3 ARTICLES PUBLISHED IN JoVE

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Biology

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Haseong Kim 1, Kil Koang Kwon 1, Wonjae Seong 1, Seung-Goo Lee 1,2
1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, South Korea

This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).

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Biochemistry

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Haseong Kim *1, Gui Hwan Han *1, Yaoyao Fu 1, Jongsik Gam 2, Seung Goo Lee 1,3
1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2College of Interdisciplinary & Creative Studies, Konyang University, 3Biosystems and Bioengineering Program, University of Science and Technology

This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

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Genetics

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
Gajendra W. Suryawanshi *1,2, Song Xu *3, Yiming Xie 1, Tom Chou 3, Namshin Kim 4, Irvin S. Y. Chen 1,5, Sanggu Kim 6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)

This manuscript describes the experimental procedure and software analysis for a bidirectional integration site assay that can simultaneously analyze upstream and downstream vector-host junction DNA. Bidirectional PCR products can be used for any downstream sequencing platform. The resulting data are useful for a high-throughput, quantitative comparison of integrated DNA targets.

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