The overall goal of this methodology is to evaluate a multiple enzymes using a single high-throughput screening system. This method can help answer key questions in the microorganism and bioengineering field about metagenomic screening and enzyme
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This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).