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University of North Texas

6 ARTICLES PUBLISHED IN JoVE

Bioengineering

Demonstrating the Uses of the Novel Gravitational Force Spectrometer to Stretch and Measure Fibrous Proteins

¹Department of Biological Sciences, University of North Texas

This is a step-by step guide showing the purpose, operation, and representative results from the novel gravitational force spectrometer.

Biology

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

Anastacia M. Garcia¹, Mary L. Ladage¹, Pamela A. Padilla¹

¹Department of Biological Sciences, University of North Texas

Described here is an in vivo technique to image sub-cellular structures in animals exposed to anoxia using a gas flow through microincubation chamber in conjunction with a spinning disc confocal microscope. This method is straightforward and flexible enough to suit a variety of experimental parameters and model systems.

Immunology and Infection

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Brian K. McFarlin¹, Adam S. Venable¹, Eric A. Prado¹, Andrea L. Henning¹, Randall R. Williams¹

¹Applied Physiology Laboratory, University of North Texas

This method demonstrates a technique for assessing granulocyte function by simultaneously measuring phagocytosis of bacteria and oxidative burst. Image-based flow cytometry allowed for the identification of three distinct subsets of activated granulocytes that all differed in their relative functional capacity.

Engineering

Atomically Traceable Nanostructure Fabrication

Josh B. Ballard¹, Don D. Dick², Stephen J. McDonnell³, Maia Bischof⁴, Joseph Fu⁵, James H. G. Owen¹, William R. Owen¹, Justin D. Alexander¹, David L. Jaeger⁴, Pradeep Namboodiri⁵, Ehud Fuchs¹, Yves J. Chabal³, Robert M. Wallace³, Richard Reidy⁴, Richard M. Silver⁵, John N. Randall¹, James Von Ehr¹

¹Zyvex Labs, ²Department of Physics, University of Texas at Dallas, ³Department of Materials Science and Engineering, University of Texas at Dallas, ⁴Materials Science and Engineering, University of North Texas, ⁵National Institute of Standards and Technology

We report a protocol for combining the atomic metrology of the Scanning Tunneling Microscope for surface patterning with selective Atomic Layer Deposition and Reactive Ion Etching. Using a robust process involving numerous atmospheric exposures and transport, 3D nanostructures with atomic metrology are fabricated.

JoVE Core

Indirect Fabrication of Lattice Metals with Thin Sections Using Centrifugal Casting

Jiwon Mun¹, Jaehyung Ju¹, James Thurman²

¹Department of Mechanical and Energy Engineering, University of North Texas, ²Department of Studio Art, University of North Texas

An indirect additive manufacturing method combining a 3D printing of polymers with a centrifugal casting is outlined for manufacturing 3D octet truss metals (Al and Cu alloys) having a unit cell length of 5 mm with a wall thickness of 0.5 mm.

Behavior

Gathering Self-Initiated Rat Behavioral Data to Characterize Post-Stroke Deficits

Jared Armshaw^*1, Grayson Butcher^*1, April Becker¹

¹Department of Behavior Analysis, College of Health and Public Service, University of North Texas

A system for acquiring data from self-initiated individual behavior sessions within a social colony cage setting is presented. The efficacy of this system is demonstrated using an automated skilled reach assessment, enabling the characterization of post-stroke motor impairments, potential behavioral alterations related to motivation, circadian variations, and other innovative dependent variables.