This work was funded in part by the Beatrice H. Barrett endowment for research on neuro-operant relations to the University of North Texas (UNT). We are grateful for the input and assistance of all members of the Neuroplasticity and Repair Laboratory, especially Valerie Rojas, Mary Kate Moore, Cameron Scallon, and Hannah McGee.

All procedures and animal care were approved by the University of North Texas institutional animal care and use committee (IACUC) and adhered to National Institutes of Health guide for the care and use of Laboratory animals. Adult male and female Long-Evans rats (400-800 g, 1.5 years old), used in the present study, were housed in colony caging.
1. Equipment preparation
<ol>
	<li>Obtain or assemble the one-rat turnstile (ORT) according to the design files and instructions fo...

<ol>
	<li>Whishaw, I. Q., Kolb, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The behavior of the laboratory rat: A handbook with tests. , (2004).</li><li>Sorge, R. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Olfactory+exposure+to+males,+including+men,+causes+stress+and+related+analgesia+in+rodents.">Olfactory exposure to males, including men, causes stress and related analgesia in rodents.</a> Nature Methods. 11 (6), 629-632 (2014).</li><li>Ottesen, J. L., Weber, A., G&#252;rtler, H., Mikkelsen, L. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+housing+conditions:+Improving+the+welfare+of+experimental+animals.">New housing conditions: Improving the welfare of experimental animals.</a> Alternatives to Laboratory Animals. 32 (Suppl 1B), 397-404 (2004).</li><li>Arakawa, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ethological+approach+to+social+isolation+effects+in+behavioral+studies+of+laboratory+rodents.">Ethological approach to social isolation effects in behavioral studies of laboratory rodents.</a> Behavioural Brain Research. 341, 98-108 (2018).</li><li>Simpson, J., Kelly, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+impact+of+environmental+enrichment+in+laboratory+rats-behavioural+and+neurochemical+aspects.">The impact of environmental enrichment in laboratory rats-behavioural and neurochemical aspects.</a> Behavioural Brain Research. 222 (1), 246-264 (2011).</li><li>Van Praag, H., Kempermann, G., Gage, F. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+consequences+of+enviromental+enrichment.">Neural consequences of enviromental enrichment.</a> Nature Reviews Neuroscience. 1 (3), 191-198 (2000).</li><li>Hays, S. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+isometric+pull+task:+A+novel+automated+method+for+quantifying+forelimb+force+generation+in+rats.">The isometric pull task: A novel automated method for quantifying forelimb force generation in rats.</a> Journal of Neuroscience Methods. 212 (2), 329-337 (2013).</li><li>Wong, C. C., Ramanathan, D. S., Gulati, T., Won, S. J., Ganguly, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+automated+behavioral+box+to+assess+forelimb+function+in+rats.">An automated behavioral box to assess forelimb function in rats.</a> Journal of Neuroscience Methods. 246, 30-37 (2015).</li><li>Sindhurakar, A., Butensky, S. D., Carmel, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automated+forelimb+tasks+for+rodents:+Current+advantages+and+limitations,+and+future+promise.">Automated forelimb tasks for rodents: Current advantages and limitations, and future promise.</a> Neurorehabilitation and Neural Repair. 33 (7), 503-512 (2019).</li><li>Sindhurakar, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+automated+test+of+rat+forelimb+supination+quantifies+motor+function+loss+and+recovery+after+corticospinal+injury.">An automated test of rat forelimb supination quantifies motor function loss and recovery after corticospinal injury.</a> Neurorehabilitation and Neural Repair. 31 (2), 122-132 (2017).</li><li>Gallistel, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Screening+for+learning+and+memory+mutations:+A+new+approach.">Screening for learning and memory mutations: A new approach.</a> Acta psychologica Sinica. 42 (1), 138 (2010).</li><li>Fenrich, K. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+single+pellet+grasping+using+automated+ad+libitum+full-time+training+robot.">Improved single pellet grasping using automated ad libitum full-time training robot.</a> Behavioural Brain Research. 281, 137-148 (2015).</li><li>Brenneis, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automated+tracking+of+motion+and+body+weight+for+objective+monitoring+of+rats+in+colony+housing.">Automated tracking of motion and body weight for objective monitoring of rats in colony housing.</a> Journal of the American Association for Laboratory Animal Science. 56 (1), 18-31 (2017).</li><li>Pereira, T. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sleap:+A+deep+learning+system+for+multi-animal+pose+tracking.">Sleap: A deep learning system for multi-animal pose tracking.</a> Nature Methods. 19 (4), 486-495 (2022).</li><li>Lauer, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multi-animal+pose+estimation,+identification+and+tracking+with+deeplabcut.">Multi-animal pose estimation, identification and tracking with deeplabcut.</a> Nature Methods. 19 (4), 496-504 (2022).</li><li>Butcher, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+apparatus+for+automatically+training+and+collecting+individualized+behavioral+data+with+socially+housed+rodents.">An apparatus for automatically training and collecting individualized behavioral data with socially housed rodents.</a> Journal of Neuroscience Methods. 365, 109387 (2022).</li><li>Winter, Y., Schaefers, A. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+sorting+system+with+automated+gates+permits+individual+operant+experiments+with+mice+from+a+social+home+cage.">A sorting system with automated gates permits individual operant experiments with mice from a social home cage.</a> Journal of Neuroscience Methods. 196 (2), 276-280 (2011).</li><li>Rivalan, M., Munawar, H., Fuchs, A., Winter, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+automated,+experimenter-free+method+for+the+standardised,+operant+cognitive+testing+of+rats.">An automated, experimenter-free method for the standardised, operant cognitive testing of rats.</a> PLOS One. 12 (1), e0169476 (2017).</li><li>Deacon, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Housing,+husbandry+and+handling+of+rodents+for+behavioral+experiments.">Housing, husbandry and handling of rodents for behavioral experiments.</a> Nature Protocols. 1 (2), 936-946 (2006).</li><li>Lang, C. E., Lohse, K. R., Birkenmeier, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dose+and+timing+in+neurorehabilitation:+Prescribing+motor+therapy+after+stroke.">Dose and timing in neurorehabilitation: Prescribing motor therapy after stroke.</a> Current Opinion in Neurology. 28 (6), 549 (2015).</li><li>Butcher, G., Becker, A., Davidson, A., Baltazar, M., Armshaw, J., Cruz, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inventing+a+supercage+for+rats.">Inventing a supercage for rats.</a> , (2019).</li><li>Davidson, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+an+enriched+environment+operant+chamber+and+its+implications.">Engineering an enriched environment operant chamber and its implications.</a> , (2019).</li><li>Windle, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+analysis+of+four+different+methods+of+producing+focal+cerebral+ischemia+with+endothelin-1+in+the+rat.">An analysis of four different methods of producing focal cerebral ischemia with endothelin-1 in the rat.</a> Experimental Neurology. 201 (2), 324-334 (2006).</li><li>Reppucci, C. J., Veenema, A. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+social+versus+food+preference+test:+A+behavioral+paradigm+for+studying+competing+motivated+behaviors+in+rodents.">The social versus food preference test: A behavioral paradigm for studying competing motivated behaviors in rodents.</a> MethodsX. 7, 101119 (2020).</li><li>Borland, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+operant+task+to+assess+social+reward+and+motivation+in+rodents.">A novel operant task to assess social reward and motivation in rodents.</a> Journal of Neuroscience Methods. 287, 80-88 (2017).</li><li>Tzschentke, T. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Review+on+cpp:+Measuring+reward+with+the+conditioned+place+preference+(cpp)+paradigm:+Update+of+the+last+decade.">Review on cpp: Measuring reward with the conditioned place preference (cpp) paradigm: Update of the last decade.</a> Addiction Biology. 12 (3-4), 227-462 (2007).</li><li>Salamone, J. D., Correa, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurobiology+and+pharmacology+of+activational+and+effort-related+aspects+of+motivation:+Rodent+studies.">Neurobiology and pharmacology of activational and effort-related aspects of motivation: Rodent studies.</a> Current Opinion in Behavioral Sciences. 22, 114-120 (2018).</li><li>Shull, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bouts,+changeovers,+and+units+of+operant+behavior.">Bouts, changeovers, and units of operant behavior.</a> European Journal of Behavior Analysis. 12 (1), 49-72 (2011).</li><li>Gottlieb, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+bidirectional+impact+of+sleep+and+circadian+rhythm+dysfunction+in+human+ischaemic+stroke:+A+systematic+review.">The bidirectional impact of sleep and circadian rhythm dysfunction in human ischaemic stroke: A systematic review.</a> Sleep Medicine Reviews. 45, 54-69 (2019).</li><li>Lo, E. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circadian+biology+and+stroke.">Circadian biology and stroke.</a> Stroke. 52 (6), 2180-2190 (2021).</li><li>Meng, H., Liu, T., Borjigin, J., Wang, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ischemic+stroke+destabilizes+circadian+rhythms.">Ischemic stroke destabilizes circadian rhythms.</a> Journal of Circadian Rhythms. 6 (1), 1-13 (2008).</li><li>Stern, R. A., Bachman, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Depressive+symptoms+following+stroke.">Depressive symptoms following stroke.</a> The American Journal of Psychiatry. 148 (3), 351-356 (1991).</li><li>Rapolien&#279;, J., Endzelyt&#279;, E., Jasevi&#269;ien&#279;, I., Savickas, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stroke+patients+motivation+influence+on+the+effectiveness+of+occupational+therapy.">Stroke patients motivation influence on the effectiveness of occupational therapy.</a> Rehabilitation Research and Practice. 2018, (2018).</li><li>Robinson, R. G., Jorge, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Post-stroke+depression:+A+review.">Post-stroke depression: A review.</a> American Journal of Psychiatry. 173 (3), 221-231 (2016).</li><li>Faraji, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sex-specific+stress+and+biobehavioral+responses+to+human+experimenters+in+rats.">Sex-specific stress and biobehavioral responses to human experimenters in rats.</a> Frontiers in Neuroscience. 16, 965500 (2022).</li></ol>

This protocol has multiple uses. First, and most broadly, the ORT was developed for the purpose of enabling automated single-subject behavioral training and data collection in the context of social, enriched housing. While this study tested the idea of collecting typical behavioral measures and elaborating upon them in the context of stroke, the same can be done for other applications and behavioral tasks. Even the measures gathered in this validation can also be adjusted as needed to include alternative reinforcement sc...

Behavioral training and testing with rat models are important in countless research areas, from the exploration of cognitive processes to disease states and more1. Typically, this training and testing is conducted with single animals in one-on-one sessions, with a researcher manually removing the animal from their home caging and temporarily placing them in some kind of apparatus. Unfortunately, there are several difficulties and limitations with this approach. First, behavioral testing can take a great deal of time for researchers, and when training is necessary, that time requirement becomes even greater. Second, this approach automatically affects-or even potentially confounds-the acquired data, as has been established elsewhere2. These confounds are especially salient when considering enrichment-related variables. Specifically, laboratory rats are traditionally housed in small cages that are just big enough for one or two rats3, and if running wheels are not provided, they may go a lifetime without meaningful opportunities to exercise. Additionally, isolated housing can be a major source of stress in a social species such as the rat4. Some of these welfare-related drawbacks likely impact rat physiology5,6, which may preempt the development of species-typical behavioral expression4 and impact the quality of rodent models as applied to human contexts.
Researchers have pursued several types of solutions to these problems in recent years. The simplest type of solution has been to automate behavioral testing and training7,8,9,10, thus removing the requirement for a single researcher to attend to a single animal. An additional solution has been to automate animal transfer to experimental chambers11,12, further removing the need for human involvement. Last, several setups have been explored which allow animals to be housed in colony caging with other animals and with more room for exploration and enrichment13. Despite these advantages, such colony setups can limit or complicate the efforts to gather individually differentiated behavioral data (though see efforts to use computer vision)14,15. If individual behavioral data is required, it can be more difficult or complex to identify and retrieve animals from colony caging for behavioral sessions as well. At present, few systems exist for collecting individual behavioral data from (enriched) colony housing16,17,18.
These drawbacks may specifically impact research on the behavioral effects of acquired brain injury. First, it is clear that the presence and/or sex of humans as well as handling practices affect rodent behavior2,19, and these variables may differentially impact the behavior of rats before vs. after stroke. Second, human behavioral outcomes after stroke can be worsened by voluntarily decreased engagement with the recommended dosage of rehabilitation exercises20. Currently, rodent experiments tend to not model this sort of context, because rats are not free to choose to engage or abstain from behavioral sessions.
This article introduces a protocol designed to facilitate individual behavioral testing within the framework of enriched colony caging. This approach not only addresses the constraints of current practices but also opens avenues for the exploration of innovative measures. A one-rat turnstile (ORT) has been developed and can be affixed to a colony cage, enabling animals to enter behavioral chambers independently and initiate their own training and testing sessions. The system is affordable; each ORT can be assembled at low cost (given access to a 3D printer). In the past, validation of this system was carried out using a basic operant chamber, showing that animals could be consistently trained to perform a simple operant lever press without the presence of an experimenter16. Nevertheless, the question of whether this configuration is applicable to other scenarios remains unresolved. The aim is to validate the effectiveness of the ORT-colony caging setup, which was previously established, for training and quantifying skilled reach behavior relevant to motor impairment following a stroke. The configuration was utilized to generate novel variables that are typically not explored in stroke research. These variables include performance metrics for the skilled reach task and measurements of self-initiation, which could be pertinent to motivation and decision-making. Furthermore, stroke-induced changes in the circadian patterns of daily self-initiation across the entire 24 h period were effectively detected.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3D printer&#160;</td>
			<td></td>
			<td></td>
			<td>Consult with local makerspace</td>
		</tr>
		<tr>
			<td>bolt</td>
			<td>Boltdepot</td>
			<td>1346</td>
			<td>6-32 or 8-32 by&#160; 0.5&#34;</td>
		</tr>
		<tr>
			<td>bolt</td>
			<td>Boltdepot</td>
			<td>1348</td>
			<td>6-32 or 8-32 by&#160; 0.75&#34;</td>
		</tr>
		<tr>
			<td>door hinge</td>
			<td>XJS (Amazon)</td>
			<td>43398-16234</td>
			<td>1&#34; cabinet stainless steel door hinge set; Optional (if &#34;perfect hinge&#34; is not printed)</td>
		</tr>
		<tr>
			<td>drill</td>
			<td></td>
			<td></td>
			<td>Any electric drill works</td>
		</tr>
		<tr>
			<td>extension spring</td>
			<td>Nieko (Amazon)</td>
			<td>50456A</td>
			<td>Choose and adjust spring based on ORT sized and desired tension</td>
		</tr>
		<tr>
			<td>granulated sugar</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>lock nuts</td>
			<td>Boltdepot</td>
			<td>2551</td>
			<td>6-32 or 8-32</td>
		</tr>
		<tr>
			<td>measuring tape</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>microcontroller</td>
			<td>Arduino</td>
			<td>A000066</td>
			<td>Arduino Uno</td>
		</tr>
		<tr>
			<td>microswitch</td>
			<td>Sparkfun</td>
			<td>KW4-Z5F</td>
			<td>mini microswitch (SPDT-roller lever)</td>
		</tr>
		<tr>
			<td>One Rat Turnstile (ORT)</td>
			<td>Vulintus</td>
			<td></td>
			<td>Contact company to request quote if not self-assembling</td>
		</tr>
		<tr>
			<td>Operant Chambers as desired for behavioral assessment: For this experiment we used automated isometric pull chambers from Vulintus&#160;</td>
			<td>Vulintus</td>
			<td>No cat #: contact Vulintus</td>
			<td>Contact Vulintus for quote</td>
		</tr>
		<tr>
			<td>PLA filament&#160;</td>
			<td>OVERTURE (Amazon)</td>
			<td>UK-MATTEPLA17511</td>
			<td></td>
		</tr>
		<tr>
			<td>plexiglass</td>
			<td>Lesnlok (Amazon)</td>
			<td>B09P74K7BR</td>
			<td>clear, 1/8&#34; thickness, Cut to size</td>
		</tr>
		<tr>
			<td>plexiglass cutter</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>python program</td>
			<td>Python Software Foundation</td>
			<td></td>
			<td>software available on request</td>
		</tr>
		<tr>
			<td>RFID reader</td>
			<td>Priority 1 Design</td>
			<td>RFIDRW-E-USB</td>
			<td>With antenna</td>
		</tr>
		<tr>
			<td>RFID tag</td>
			<td>Unified Information Devices</td>
			<td>UC-1485-10</td>
			<td></td>
		</tr>
		<tr>
			<td>rod</td>
			<td>Boltdepot</td>
			<td>23632</td>
			<td>cut to &#62; 3.5&#34;</td>
		</tr>
		<tr>
			<td>Rotary tool</td>
			<td></td>
			<td></td>
			<td>Used to bore hole in apparatus and colony caging for ORT; any hardware usable</td>
		</tr>
		<tr>
			<td>sand paper</td>
			<td>HSYMQ (Amazon)</td>
			<td>TOMPOL-1118-1915-11</td>
			<td></td>
		</tr>
		<tr>
			<td>socket wrench set</td>
			<td></td>
			<td></td>
			<td>Any socket wrench set works</td>
		</tr>
		<tr>
			<td>soldering iron</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>super glue</td>
			<td></td>
			<td>234790</td>
			<td></td>
		</tr>
		<tr>
			<td>wire</td>
			<td>Plusivo (Amazon)</td>
			<td>EAN0721248989789</td>
			<td></td>
		</tr>
	</tbody>
</table>

gathering self-initiated rat behavioral data to characterize post-stroke deficits

Behavioral testing in rat models is frequently utilized for diverse purposes, including psychological, biomedical, and behavioral research. Many traditional approaches involve individual, one-on-one testing sessions between a single researcher and each animal in an experiment. This setup can be very time consuming for the researcher, and their presence may impact the behavioral data in unwanted ways. Additionally, traditional caging for rat research imposes a lack of enrichment, exercise, and socialization that would normally be typical for the species, and this context may also skew the results of behavioral data. Overcoming these limitations may be worthwhile for several research applications, including the study of acquired brain injury. Here, an example method is presented for automatically training and testing individual rat behavior in a colony cage without the presence of humans. Radio frequency identification can be utilized to tailor sessions to the individual rat. The validation of this system occurred in the example context of measuring skilled forelimb motor performance before and after stroke. Traditional characteristics of post-stroke behavioral impairments and novel measures enabled by the system are measured, including success rate, various aspects of pull force, bout analysis, initiation rate and patterns, session duration, and circadian patterns. These variables can be collected automatically with few limitations; though the apparatus removes experimental control of exposure, timing and practice, the validation produced reasonable consistency in these variables from animal to animal.

The animals were trained and tested with four female rats in one colony cage and four male rats in a separate colony cage. All rats learned to pass through the ORTs in four days or less. The four female rats reached &#62;85% successful bouts at the 120 g force requirement in approximately 6 weeks of training and the male rats reached the same criterion in 10 weeks (compared to roughly 3 weeks with standard training with deprived rats)7. This training duration was greatly lengthened due to several ...

Watch this Scientific Journal Video about Gathering Self-Initiated Rat Behavioral Data to Characterize Post-Stroke Deficits at JoVE.com

Gathering Self-Initiated Rat Behavioral Data to Characterize Post-Stroke Deficits

A system for acquiring data from self-initiated individual behavior sessions within a social colony cage setting is presented. The efficacy of this system is demonstrated using an automated skilled reach assessment, enabling the characterization of post-stroke motor impairments, potential behavioral alterations related to motivation, circadian variations, and other innovative dependent variables.

Behavioral testing in rat models is frequently utilized for diverse purposes, including psychological, biomedical, and behavioral research. Many ...

gathering-self-initiated-rat-behavioral-data-to-characterize-post

Research

JoVE Journal

Behavior

775 vistas.  University of North Texas. Esta investigación tiene como objetivo abordar las limitaciones típicas en los experimentos basados en el comportamiento al permitir que los animales vivan en establos enriquecidos. El objetivo es facilitar las pruebas y el entrenamiento conductual autoiniciados, y demostrar cómo ese enfoque puede generar datos individualizados válidos, incluidas las variables dependientes posteriores al accidente cerebrovascular, tanto típicas como novedosas. Existen varios desafíos para los investigad...