Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor LibrariesElijah W. Chen *1, Chyan Ying Ke *2,3, Joanna Brzostek *1, Nicholas R. J. Gascoigne 1, Vasily Rybakin 1,4
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven
This paper uses a flow-cytometry-based assay to screen libraries of chemical inhibitors for the identification of inhibitors and their targets that influence T-cell receptor signaling. The methods described here can also be expanded for high-throughput screenings.
