Masonic Medical Research Institute
2 ARTICLES PUBLISHED IN JoVE
Medicine
Ultrasound-guided Transthoracic Intramyocardial Injection in Mice
Terence W. Prendiville 1, Qing Ma 1, Zhiqiang Lin 1, Pingzhu Zhou 1, Aibin He 1, William T. Pu 1,2
1Department of Cardiology, Boston Children's Hospital, 2Harvard Stem Cell Institute, Harvard University
Echocardiography-guided percutaneous intramyocardial injection represents an efficient, reliable, and targetable modality for the delivery of gene transfer agents or cells into the murine heart. Following the steps outlined in this protocol, the operator can quickly become competent in this versatile, minimally invasive technique.
Immunology and Infection
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
Steven G. Negron 1, Bing Xu 1, Zhiqiang Lin 1
1Masonic Medical Research Institute
This study describes a new method of isolating murine brown adipocytes for gene and protein expression analysis.
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