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Masonic Medical Research Institute

2 ARTICLES PUBLISHED IN JoVE

Medicine

Ultrasound-guided Transthoracic Intramyocardial Injection in Mice

Terence W. Prendiville¹, Qing Ma¹, Zhiqiang Lin¹, Pingzhu Zhou¹, Aibin He¹, William T. Pu^1,2

¹Department of Cardiology, Boston Children's Hospital, ²Harvard Stem Cell Institute, Harvard University

Echocardiography-guided percutaneous intramyocardial injection represents an efficient, reliable, and targetable modality for the delivery of gene transfer agents or cells into the murine heart. Following the steps outlined in this protocol, the operator can quickly become competent in this versatile, minimally invasive technique.

Immunology and Infection

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Steven G. Negron¹, Bing Xu¹, Zhiqiang Lin¹

¹Masonic Medical Research Institute

This study describes a new method of isolating murine brown adipocytes for gene and protein expression analysis.