Louisiana State University Health-School of Dentistry
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
Junko Yano 1, Paul L. Fidel, Jr. 1
1Louisiana State University Health Sciences Center
Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.
Immunology and Infection
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
Junko Yano 1, Elizabeth A. Lilly 2, Mairi C. Noverr 2, Paul L. Fidel 1
1Center for Oral and Craniofacial Biology, Louisiana State University Health-School of Dentistry, 2Department of Microbiology and Immunology, Tulane University School of Medicine
Here, we present an effective and efficient method for rodent tail vein injections using a uniquely designed warming/restraining device. By streamlining the initiation of vasodilation and restraining processes, this protocol allows accurate and timely intravenous injections of large groups of animals with minimal distress.
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