Louisiana State University Health-School of Dentistry

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

¹Louisiana State University Health Sciences Center

Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.

Immunology and Infection

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

Junko Yano¹, Elizabeth A. Lilly², Mairi C. Noverr², Paul L. Fidel¹

¹Center for Oral and Craniofacial Biology, Louisiana State University Health-School of Dentistry, ²Department of Microbiology and Immunology, Tulane University School of Medicine

Here, we present an effective and efficient method for rodent tail vein injections using a uniquely designed warming/restraining device. By streamlining the initiation of vasodilation and restraining processes, this protocol allows accurate and timely intravenous injections of large groups of animals with minimal distress.