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Idaho College of Osteopathic Medicine

3 ARTICLES PUBLISHED IN JoVE

Biology

Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes

Michelle Z. Chiu^1,2, Steven Lannon¹, Marisol Luchetti¹, Michael B. Wells³, Deborah J. Andrew¹

¹Department of Cell Biology, The Johns Hopkins University School of Medicine, ²Tufts School of Medicine, ³Department of Biomedical Sciences, Idaho College of Osteopathic Medicine

The adult mosquito salivary gland (SG) is required for the transmission of all mosquito-borne pathogens to their human hosts, including viruses and parasites. This video demonstrates efficient isolation of the SGs from the larval (L4) stage Anopheles gambiae mosquitoes and preparation of the L4 SGs for further analysis.

Biology

Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes

Mabel Taracena^1,2, Catherine Hunt¹, Pamela Pennington³, Deborah Andrew^4,5, Marcelo Jacobs-Lorena^5,6, Ellen Dotson¹, Michael Wells^5,7,8

¹Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, ²Department of Entomology, Cornell University, ³Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, ⁴Department of Cell Biology, Johns Hopkins School of Medicine, ⁵Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, ⁶Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, ⁷Department of Cell Biology, Johns Hopkins School of Medicine, ⁸Biomedical Sciences Department, Idaho College of Osteopathic Medicine

The oral administration of dsRNA produced by bacteria, a delivery method for RNA interference (RNAi) that is routinely used in Caenorhabditis elegans, was successfully applied here to adult mosquitoes. Our method allows for robust reverse genetics studies and transmission-blocking vector studies without the use of injection.

Highlighting Molecular Biology as a Focal Point for Mosquito Research

Michael B. Wells¹

¹Department of Biomedical Sciences, Idaho College of Osteopathic Medicine

Highlighting Molecular Biology as a Focal Point for Mosquito Research