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4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Mia I. Temkin¹, Caitlin M. Carlson¹, Aaron L. Stubbendieck², Cameron R. Currie¹, Reed M. Stubbendieck¹

¹Department of Bacteriology, University of Wisconsin-Madison, ²Independent Scholar

The co-culture interaction assays presented in this protocol are inexpensive, high throughput, and simple. These assays can be used to observe microbial interactions in co-culture, identify interaction patterns, and characterize the inhibitory potential of a microbial strain of interest against human and environmental pathogens.

Medicine

Developing and Testing Methylated Nano-Structured Dipeptides that Inhibit Src Kinase Activity In Vitro for Anti-Cancer Applications

Alaa F. Nahhas¹, Alrayan F. Nahhas², Thomas J. Webster^3,4,5

¹Biochemistry Department, College of Science, King Abdulaziz University, ²Independent Scholar, ³School of Health Sciences and Biomedical Engineering, Hebei University of Technology, ⁴School of Engineering, Saveetha University, ⁵Programa de Pós-graduação em Ciência e Engenharia dos Materiais, UFPI

Presented here is a protocol for designing and testing dipeptides that can inhibit Src kinase enzyme activity using acellular and cellular assays for anti-cancer applications. The peptides formulated here (W-R_CH3, W_CH3-R_CH3, and W-R_(CH3)2) inhibited Src kinase with IC₅₀ values of 510 nM, 916 nM, and 1 µM, respectively.

Environment

Dendrochronological Dating and Provenancing of String Instruments

Katarina Čufar¹, Blaž Demšar², Micha Beuting², Angela Balzano¹, Nina Škrk¹, Luka Krže¹, Maks Merela¹

¹Department of Wood Science and Technology, Biotechnical Faculty, University of Ljubljana, ²Independent Scholar

The dendrochronological analysis of a stringed instrument requires inspecting the top plate, measuring the tree ring widths, establishing the chronology of the instrument, and dating through determining the end date-the year of the formation of the most recent tree ring.

Biology

Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

Michael Hamersky IV¹, Khushi Tekale¹, L. Matthew Winfree², Matthew J. M. Rowan^1,3, Lindsey Seldin^1,4,5,6

¹Department of Cell Biology, Emory University School of Medicine, ²Independent scholar, ³Center for Neurodegenerative Disease, Emory University School of Medicine, ⁴Department of Dermatology, Emory University School of Medicine, ⁵Winship Cancer Institute, Emory University School of Medicine, ⁶Atlanta Veterans Affairs Medical Center

The protocol presents a new tool to simplify intravital imaging using inverted confocal microscopy.