Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass SpectrometryJessica L. Nickerson 1, Venus Baghalabadi 1, Ziheng Dang 1, Victoria A. Miller 2, Sara L. Little 2, Alan A. Doucette 1
1Department of Chemistry, Dalhousie University, 2Proteoform Scientific Inc.
The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.
