S'identifier

Proteoform Scientific Inc.

1 ARTICLES PUBLISHED IN JoVE

JoVE Core

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Jessica L. Nickerson¹, Venus Baghalabadi¹, Ziheng Dang¹, Victoria A. Miller², Sara L. Little², Alan A. Doucette¹

¹Department of Chemistry, Dalhousie University, ²Proteoform Scientific Inc.

The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.