Amgen
2 ARTICLES PUBLISHED IN JoVE
JoVE Core
Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System
Jonathan E. Phillips 1, Xuxia Zhang 1, James A. Johnston 1
1Inflammation Research, Amgen
The inhalation unit described herein can generate, sample for characterization, and uniformly deposit a drug aerosol in the lungs of rodents. This enables the pre-clinical determination of the efficacy and safety of drug doses deposited in the lungs; key data enabling clinical inhaled drug development.
Genetics
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Nicole A. Vander Schaaf 1, Shirley Oghamian 2, Jin-A Park 1, Liang Kang 1, Peter W. Laird 1, Kwang-Ho Lee 1
1Center for Epigenetics, Van Andel Research Institute, 2Amgen
This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems.
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