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North Dakota State University

4 ARTICLES PUBLISHED IN JoVE

Biology

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

¹Environmental and Conservation Sciences Program, North Dakota State University, ²Department of Biological Sciences, North Dakota State University

This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

Environment

A Precise and Autonomous System for the Detection of Insect Emergence Patterns

Meghan M. Bennett^1,2, Joseph P. Rinehart³, George D. Yocum³, Ian Yocum³

¹Department of Biological Sciences, North Dakota State University, ²School of Life Sciences, Arizona State University, ³Red River Valley Agricultural Research Center, USDA-ARS

Measurement of insect emergence patterns requires precision. Existing systems are only semi-automated and sample size is limited. We addressed these issues by designing a system using microcontrollers to precisely measure the time of emergence of large numbers of emerging insects.

Bioengineering

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Meihua Wan^1,2, Pouya Amrollahi^2,3, Dali Sun⁴, Christopher Lyon^2,3, Tony Y. Hu^2,3

¹Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, ²Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, ³School of Biological and Health Systems Engineering, Arizona State University, ⁴Department of Electrical and Computer Engineering, North Dakota State University

Clinical translation of exosome-derived biomarkers for diseased and malignant cells is hindered by the lack of rapid and accurate quantification methods. This report describes the use of low-magnification dark-field microscope images to quantify specific exosome subtypes in small volume serum or plasma samples.

Bioengineering

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Bharat Kumar Reddy Sanapalli¹, Kalaivani Chinna Gounder¹, Nilesh Sudhakar Ambhore², Gowthamarajan Kuppuswamy¹, Praveen Thaggikuppe Krishnamurthy³, Veera Venkata Satyanarayana Reddy Karri¹

¹Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education & Research, ²Department of Pharmaceutical Sciences, School of Pharmacy, North Dakota state university, ³Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education & Research

The prepared DOX-CL scaffold satisfied the prerequisites of an ideal DW dressing in mechanical strength, porosity, water absorption, degradation rate, sustained release, anti-bacterial, biocompatibility, and anti-inflammatory properties, which are considered to be essential for the recovery of damaged tissue in DWs.