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University Hospital of Cologne, Department I of Internal Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Generation of Human CD40-activated B cells

Tanja M. Liebig¹, Anne Fiedler¹, Shahram Zoghi¹, Alexander Shimabukuro-Vornhagen¹, Michael S. von Bergwelt-Baildon¹

¹Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program, University Hospital of Cologne, Department I of Internal Medicine

In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.

Immunology and Infection

Murine Model of CD40-activation of B cells

Tanja M. Liebig¹, Anne Fiedler¹, Nela Klein-Gonzalez¹, Alexander Shimabukuro-Vornhagen¹, Michael von Bergwelt-Baildon¹

¹Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine, University Hospital of Cologne

In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.