University Hospital of Cologne, Department I of Internal Medicine
2 ARTICLES PUBLISHED IN JoVE
Biology
Generation of Human CD40-activated B cells
Tanja M. Liebig 1, Anne Fiedler 1, Shahram Zoghi 1, Alexander Shimabukuro-Vornhagen 1, Michael S. von Bergwelt-Baildon 1
1Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program, University Hospital of Cologne, Department I of Internal Medicine
In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.
Immunology and Infection
Murine Model of CD40-activation of B cells
Tanja M. Liebig 1, Anne Fiedler 1, Nela Klein-Gonzalez 1, Alexander Shimabukuro-Vornhagen 1, Michael von Bergwelt-Baildon 1
1Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine, University Hospital of Cologne
In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.
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