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Howard Hughes Medical Institute and Gene Expression Laboratory

4 ARTICLES PUBLISHED IN JoVE

Biology

Allyn Meyer¹, Benjamin W. Gallarda^1,2, Samuel Pfaff¹, William Alaynick¹

¹The Salk Institute for Biological Studies, Howard Hughes Medical Institute and Gene Expression Laboratory, ²Biology Graduate Program, University of California San Diego - UCSD

A demonstration of the isolation of neonatal mouse spinal cord for electrophysiologic studies.

Neuroscience

Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication

Suresh Garudadri¹, Benjamin Gallarda¹, Samuel Pfaff¹, William Alaynick¹

¹Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk Institute for Biological Studies

A demonstration of the fabrication and use of an extracellular suction electrode used to measure electrophysiological recordings of neonatal rodent spinal cords in vitro

Neuroscience

Using Affordable LED Arrays for Photo-Stimulation of Neurons

Matthew Valley¹, Sebastian Wagner¹, Benjamin W. Gallarda¹, Pierre-Marie Lledo¹

¹Laboratory for Perception and Memory, Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)

Adult-born neurons expressing ChR2 can be manipulated in slice electrophysiological preparations in order to examine their contribution towards the function of olfactory neural circuits.

Neuroscience

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Gabriel Lepousez¹, Mariana Alonso¹, Sebastian Wagner¹, Benjamin W. Gallarda¹, Pierre-Marie Lledo¹

¹Laboratory for Perception and Memory, Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)

Adult-born neurons of the olfactory bulb can be optogenetically controlled using Channelrhodopsin2-expressing lentiviral injection in the rostral migratory stream and chronic photostimulation with an implanted miniature LED.