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University of Maryland, Baltimore County

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Labeling and Imaging Cells in the Zebrafish Hindbrain
Pradeepa Jayachandran 1, Elim Hong 2, Rachel Brewster 1
1Department of Biological Sciences, University of Maryland, Baltimore County, 2Center for Neuroscience, Children's National Medical Center

Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.

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Chemistry

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography
Gregory T. Winter 1, Joshua A. Wilhide 1, William R. LaCourse 1
1Department of Chemistry and Biochemistry, University of Maryland, Baltimore County

This paper presents a protocol for the visualization of gaseous streams of an ambient ionization source using schlieren photography and mass spectrometry.

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Developmental Biology

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
Rebecca J. McFarland *1, Sharlene P. Brown *1, Eudorah Vital 1, Jonathan M. Werner 1, Rachel M. Brewster 1
1Department of Biological Sciences, University of Maryland, Baltimore County

Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.

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