Results: Analysis of Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
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Conclusion
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The overall goal of these labeling procedures is to image and quantify stable, dynamic and nascent microtubules in the developing zebrafish embryo. This method can help answer key questions in the field of developmental neurobiology such as, how m
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Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.