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INSERM U1223

6 ARTICLES PUBLISHED IN JoVE

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Advanced Biology

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
Perchet Thibaut 1,2,3, Sophie1 Novault 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

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Advanced Biology

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes
Meunier Sylvain 1,2,3, Perchet Thibaut 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

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Advanced Biology

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
Perchet Thibaut 1,2,3, Meunier Sylvain 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platform, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

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Advanced Biology

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
Meunier Sylvain 1,2,3, Perchet Thibaut 1,2,3, Sophie Novault 4, Rachel Golub 1,2,3
1Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, 2INSERM U1223, 3Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, 4Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

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Genetics

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Thibaut Perchet 1, Sylvestre Chea 1, Milena Hasan 2, Ana Cumano 1, Rachel Golub 1
1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 2Center for Translational Science, Institut Pasteur, INSERM UMS20

This protocol describes how to assess the expression of a large array of genes at the clonal level. Single-cell RT-qPCR produces highly reliable results with a strong sensitivity for hundreds of samples and genes.

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Immunology and Infection

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Alba Llibre *1,2, Vincent Bondet *1,2, Mathieu P. Rodero 3, David Hunt 4, Yanick J. Crow 3,5, Darragh Duffy 1,2
1Immunobiology of Dendritic Cells, Institut Pasteur, 2INSERM U1223, 3Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163, Institut Imagine, 4MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, 5Manchester Centre for Genomic Medicine, University of Manchester

Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples.

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