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INSERM U1223

6 ARTICLES PUBLISHED IN JoVE

Advanced Biology

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

Perchet Thibaut^1,2,3, Sophie1 Novault^1,2,3, Sophie Novault⁴, Rachel Golub^1,2,3

¹Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, ²INSERM U1223, ³Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, ⁴Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

Advanced Biology

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

Meunier Sylvain^1,2,3, Perchet Thibaut^1,2,3, Sophie Novault⁴, Rachel Golub^1,2,3

¹Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, ²INSERM U1223, ³Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, ⁴Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

Advanced Biology

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

Perchet Thibaut^1,2,3, Meunier Sylvain^1,2,3, Sophie Novault⁴, Rachel Golub^1,2,3

¹Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, ²INSERM U1223, ³Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, ⁴Flow Cytometry Platform, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

Advanced Biology

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

Meunier Sylvain^1,2,3, Perchet Thibaut^1,2,3, Sophie Novault⁴, Rachel Golub^1,2,3

¹Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, ²INSERM U1223, ³Université Paris Diderot, Sorbonne Paris Cité Cellule Pasteur, ⁴Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

Genetics

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Thibaut Perchet¹, Sylvestre Chea¹, Milena Hasan², Ana Cumano¹, Rachel Golub¹

¹Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, ²Center for Translational Science, Institut Pasteur, INSERM UMS20

This protocol describes how to assess the expression of a large array of genes at the clonal level. Single-cell RT-qPCR produces highly reliable results with a strong sensitivity for hundreds of samples and genes.

Immunology and Infection

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Alba Llibre^*1,2, Vincent Bondet^*1,2, Mathieu P. Rodero³, David Hunt⁴, Yanick J. Crow^3,5, Darragh Duffy^1,2

¹Immunobiology of Dendritic Cells, Institut Pasteur, ²INSERM U1223, ³Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163, Institut Imagine, ⁴MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, ⁵Manchester Centre for Genomic Medicine, University of Manchester

Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples.

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