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School of Veterinary Medicine, University of California - Davis

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Yoosook Lee¹, Allison M. Weakley¹, Catelyn C. Nieman¹, Julia Malvick², Gregory C. Lanzaro¹

¹Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California - Davis, ²Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California, Davis

Malaria transmitting mosquitoes have a number of epidemiologically important characteristics that can only be detected using molecular techniques. Utilizing a MALDI-TOF based SNP genotyping platform, we developed an assay for simultaneously detecting multiple key traits (species, insecticide resistance, parasite infection and host choice) of malaria vectors.

Biology

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Tse-Yu Chen¹, Adam E. Vorsino², Kyle J. Kosinski¹, Ana L. Romero-Weaver¹, Eva A. Buckner¹, Joanna C. Chiu³, Yoosook Lee¹

¹Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, ²U. S. Fish and Wildlife Service, Pacific Islands Fish and Wildlife Office, ³Department of Entomology and Nematology, University of California Davis

Described here is a DNA extraction protocol using magnetic beads to produce high quality DNA extractions from mosquitoes. These extractions are suitable for a downstream next-generation sequencing approach.