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Seoul National University College of Medicine

9 ARTICLES PUBLISHED IN JoVE

Neuroscience

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Sung Wook Park^1,2, Jin Hyoung Kim², Woo Jin Park³, Jeong Hun Kim^1,2,4

¹Department of Biomedical Sciences, Seoul National University College of Medicine, ²FARB Laboratory, Biomedical Research Institute, Seoul National University Hospital, ³College of Life Sciences, Gwangju Institute of Science and Technology, ⁴Department of Ophthalmology, Seoul National University College of Medicine

Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.

Biology

Assessment of Myofilament Ca²⁺ Sensitivity Underlying Cardiac Excitation-contraction Coupling

Zai Hao Zhao¹, Chun Li Jin¹, Ji Hyun Jang¹, Yu Na Wu¹, Sung Joon Kim¹, Hong Hua Jin², Lan Cui², Yin Hua Zhang^1,2,3

¹Department of Physiology & Biomedical Sciences, Ischemic/hypoxic Disease Institute, Seoul National University College of Medicine, ²Yan Bian University Hospital, ³Institute of Cardiovascular Sciences, University of Manchester

This paper describes a protocol that assesses the changes of myofilament Ca²⁺ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca²⁺ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts.

Behavior

Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain

Jaewon Beom^1,2, Jung Chan Lee^2,3,4, Jin Chul Paeng⁵, Tai Ryoon Han⁶, Moon Suk Bang⁷, Byung-Mo Oh⁷

¹Department of Rehabilitation Medicine, Chungnam National University Hospital, Daejeon, ²Department of Biomedical Engineering, Seoul National University College of Medicine, ³Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, ⁴Department of Biomedical Engineering, Seoul National University Hospital, ⁵Department of Nuclear Medicine, Seoul National University Hospital, Seoul National University College of Medicine, ⁶Department of Rehabilitation Medicine, Gangwon Do Rehabilitation Hospital, ⁷Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul National University College of Medicine

We applied repetitive transcranial magnetic stimulation (rTMS) to the unilateral hemisphere of rat brain, by placing a 25-mm figure-8 coil 1 cm lateral to the vertex on the biauricular line and angulating the coil by 45°. An in-house water cooling system was used for rTMS for more than 20 min.

Bioengineering

Robotic Mirror Therapy System for Functional Recovery of Hemiplegic Arms

Jaewon Beom^*1,2, Sukgyu Koh^*3, Hyung Seok Nam¹, Wonshik Kim³, Yoonjae Kim³, Han Gil Seo⁴, Byung-Mo Oh^4,5, Sun Gun Chung^4,5, Sungwan Kim^1,6

¹Department of Biomedical Engineering, Seoul National University College of Medicine, ²Department of Rehabilitation Medicine, Chungnam National University Hospital, ³Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, ⁴Department of Rehabilitation Medicine, Seoul National University Hospital, ⁵Seoul National University College of Medicine, ⁶Institute of Medical and Biological Engineering, Seoul National University

We developed a real-time mirror robot system for functional recovery of hemiplegic arms using automatic control technology, conducted a clinical study on healthy subjects, and determined tasks through feedback from rehabilitation doctors. This simple mirror robot can be applied effectively to occupational therapy in stroke patients with a hemiplegic arm.

Cancer Research

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Ji-Eun Chang¹, Hyun-Jong Cho², Sanghoon Jheon^1,3

¹Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, ²College of Pharmacy, Kangwon National University, ³Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine

Photodynamic therapy (PDT) is an alternative choice for lung cancer treatment. To increase the therapeutic effect of PDT, lung cancer-targeted nanoparticles combined with chemotherapy were developed. Both in vitro and in vivo anticancer efficacies of PDT with prepared nanoparticles were evaluated.

Cancer Research

Ultrasonographic Evaluation of Breast Cancer-related Lymphedema

Yumi Jeon¹, Jaewon Beom¹, Soyoung Ahn¹, Soo-Kyung Bok¹

¹Department of Rehabilitation Medicine, Chungnam National University Hospital (CNUH)

This study utilized ultrasonography for diagnosis and follow-up tests to confirm treatment efficacy of Progressive Resistance Exercise (PRE) in breast cancer-related lymphedema (BCRL). Ultrasonography can be applied effectively to ensure diagnosis and treatment of lymphedema.

Medicine

Two-Dimensional X-Ray Angiography to Examine Fine Vascular Structure Using a Silicone Rubber Injection Compound

Hak Chang¹, Jeong Hyun Ha¹, Seong Oh Park²

¹Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, ²Department of Plastic and Reconstructive Surgery, Hanyang University Medical Center, Hanyang University College of Medicine

This study presents a simple two-dimensional angiographic method to examine fine vascular structures using a silicone rubber injection compound and soft tissue X-ray system.

Medicine

Porcine As a Training Module for Head and Neck Microvascular Reconstruction

Mohammad Ali Alessa^*1,2, Sang Hyun Kwak^*2, Young Woo Lee², Mi-Lan Kang³, Hak-Joon Sung^3,4, Soon Hyun Ahn⁵, Eun Chang Choi², Won Shik Kim⁵

¹Department of Otolaryngology, Head and Neck Surgery, King Abdullah Medical City, ²Department of Otorhinolaryngology, Yonsei University, College of Medicine, ³Severance Biomedical Science Institute, Yonsei University, College of Medicine, ⁴The George W.Woodruff School of Mechanical Engineering, Georgia Institute of Technology, ⁵Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine

Here we present a protocol for the use of the pig superior epigastric artery perforator flap as a learning module for head and neck microvascular reconstruction.

Bioengineering

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Daehan Kim¹, Gi-Hun Lee¹, Jiheum Park², Jung Chan Lee², Joong Yull Park¹

¹Department of Mechanical Engineering, Graduate School, Chung-Ang University, ²Department of Biomedical Engineering, College of Medicine, Seoul National University

We present a motor-powered centrifugal microfluidic device that can cultivate cell spheroids. Using this device, spheroids of single or multiple cell types could be easily cocultured under high gravity conditions.