Iniciar sesión

Emory University, Atlanta, Georgia

3 ARTICLES PUBLISHED IN JoVE

image

Biology

Cryosectioning Method for Microdissection of Murine Colonic Mucosa
Attila E. Farkas 1, Christian Gerner-Smidt 1, Loukia Lili 1, Asma Nusrat 1, Christopher T. Capaldo 1
1Epithelial Pathobiology and Mucosal Inflammation Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia

Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.

image

Immunology and Infection

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model
Vicky Garcia-Hernandez 1, Philipp-Alexander Neumann 2, Stefan Koch 3,4, Renae Lyons 1, Asma Nusrat 1, Charles A. Parkos 1
1Department of Pathology, University of Michigan, 2Department of Surgery, Klinikum Rechts der Isar, Technische Universität München, 3Wallenberg Centre for Molecular Medicine, Linköping University, 4Department of Biomedical and Clinical Sciences (BKV), Linköping University

Systematic scoring of intestinal inflammation using a free computer-assisted system is a powerful tool to quantitatively compare histopathological changes in colitis models characterized by the presence of ulcers and inflammatory changes. Histological colitis score evaluation strengthens clinical observations and facilitates data interpretation.

image

Biology

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
Chithra K Muraleedharan 1, Jay Mierzwiak 1, Darius Feier 1, Asma Nusrat 1, Miguel Quiros 1
1Department of Pathology, School of Medicine, University of Michigan

In this protocol, we describe how to generate murine primary epithelial colon monolayers directly from intestinal crypts. We provide experimental approaches to generate confluent monolayers on permeable filters, confluent monolayers for scratch wound healing and biochemical studies, and sparse and confluent monolayers for immunofluorescence analysis.

JoVE Logo

Privacidad

Condiciones de uso

Políticas

Investigación

Educación

ACERCA DE JoVE

Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados