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Université Versailles St Quentin

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
Audrey Bernut 1,2, Christian Dupont 1,2, Alain Sahuquet 1, Jean-Louis Herrmann 3, Georges Lutfalla 1, Laurent Kremer 1,2
1Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS, UMR 535, Université Montpellier, 2Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé, CNRS, FRE 3689, Université Montpellier, 3Unité de Formation et de Recherche des Sciences de la Santé, EA3647-EPIM, Université Versailles St Quentin

Optically transparent zebrafish embryos are widely used to study and visualize in real time the interactions between pathogenic microorganisms and the innate immune cells. Micro-injection of Mycobacterium abscessus, combined with fluorescence imaging, is used to scrutinize essential pathogenic features such as cord formation in zebrafish embryos.

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Immunology and Infection

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
Violaine Dubois *1, Laura Laencina *1, Anouchka Bories 1, Vincent Le Moigne 1, Alexandre Pawlik 2, Jean-Louis Herrmann 1, Fabienne Girard-Misguich 1
12I, UVSQ, INSERM UMR1173, Université Paris-Saclay, 2Institut Pasteur, Unité de Pathogénomique Mycobactérienne

Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.

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