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Christian-Albrechts-University Kiel

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Production and Titering of Recombinant Adeno-associated Viral Vectors

Christina McClure^*1, Katy L. H. Cole^*1, Peer Wulff¹, Matthias Klugmann², Andrew J. Murray^1,3

¹School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, ²Translational Neuroscience Facility and Department of Physiology, School of Medical Sciences, University of New South Wales, ³Department of Biochemistry and Molecular Biophysics, Columbia University

Recombinant adeno-associated virus (rAAVs) vectors are becoming increasingly valuable for in vivo studies in animals. We describe how rAAVs can be produced in the laboratory and how these vectors can be titered to give an accurate reading of the number of infectious particles produced.

JoVE Journal

Calibrated Passive Sampling - Multi-plot Field Measurements of NH₃ Emissions with a Combination of Dynamic Tube Method and Passive Samplers

Andreas Pacholski^1,2

¹Institute of Ecology, Assoc. Institute of Environmental and Sustainable Chemistry, Leuphana University, ²Institute of Crop Science and Plant Breeding, Department of Agronomy and Crop Science, Christian-Albrechts-University Kiel

Ammonia emissions are a major threat to the environment by eutrophication, soil acidification and fine particle formation and stem mainly from agricultural sources. This method allows ammonia loss measurements in replicated field trials enabling statistical analysis of emissions and of relationships between crop development and emissions.

Neuroscience

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

Gilda Baccini¹, Sören Brandt¹, Peer Wulff¹

¹Institute of Physiology, Christian-Albrechts-University Kiel

We present a protocol to prepare acute-slices from the dorsal-intermediate hippocampus of mice. We compare this transversal preparation with coronal slicing in terms of quality of recordings and preservation of morphological features of recorded neurons.