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Children's University Hospital Tübingen

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation and Ex Vivo Culture of Vδ1⁺CD4⁺γδ T Cells, an Extrathymic αβT-cell Progenitor

Christian Welker¹, Rupert Handgretinger¹, Karin Schilbach¹

¹Deptartment of Hematology and Oncology, Children's University Hospital Tübingen

Here, we provide an optimized protocol for the isolation and cloning of the scarce T-cell entity of peripheral Vδ1⁺CD4⁺ T cells that is, as we showed recently, an extrathymic αβ T-cell progenitor. This technique allows to quantitatively isolate, clone and efficiently expand these cells in ex vivo culture.