Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell ProgenitorChristian Welker 1, Rupert Handgretinger 1, Karin Schilbach 1
1Deptartment of Hematology and Oncology, Children's University Hospital Tübingen
Here, we provide an optimized protocol for the isolation and cloning of the scarce T-cell entity of peripheral Vδ1+CD4+ T cells that is, as we showed recently, an extrathymic αβ T-cell progenitor. This technique allows to quantitatively isolate, clone and efficiently expand these cells in ex vivo culture.
